Journal: Oncology Letters
Article Title: Downregulation of Epstein-Barr virus-induced gene 3 is associated with poor prognosis of hepatocellular carcinoma after curative resection
doi: 10.3892/ol.2018.8272
Figure Lengend Snippet: Expression of EBI3 in human hepatoma cell lines and tissue samples. (A) EBI3 was detected by western blot analysis in 5 hepatoma cell lines, 20 HCC tissues and corresponding peritumoral tissues and quantitative results of the western blot analysis, GAPDH served as a loading control *P<0.05 vs. tumor. (B) Representative immunohistochemical staining of EBI3 in tissue microarrays (magnification, ×200). EBI3, Epstein-Barr virus-induced gene 3; HCC, hepatocellular carcinoma; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Article Snippet: Following immerged in 3% H 2 O 2 for 15 min, the tissue sections were incubated with the rabbit polyclonal anti-human EBI3 antibody (dilution 1:200; sc-32,868; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) overnight at 4°C.
Techniques: Expressing, Western Blot, Control, Immunohistochemical staining, Staining, Virus